DNA Clean & Concentrator-5

Cat # Name                                                                 Size

D4004 DNA Clean & Concentrator-5 (Uncapped)         200 Preps

D4003 DNA Clean & Concentrator-5 (Uncapped)         50 Preps

D4014 DNA Clean & Concentrator-5 (Capped)         200 Preps

D4013 DNA Clean & Concentrator-5 (Capped)         50 Preps

D4003T DNA Clean & Concentrator-5 (Uncapped)         10 Preps

Brand：Zymo Research

_d4003t_d4003_d4004_d4013_d4014_dna_clean_concentrator_-5.pdf

HIGHLIGHTS

Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.

Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.

Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

DESCRIPTION

The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.

TECHNICAL SRECIFICATIONS

Detergent Tolerance

≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS

Elution Volume

≥ 6 µl

Equipment

Microcentrifuge

Purity

A260/A280 > 1.8

Sample Source

DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.

Size Range

50 bp to 23 kb

Yield

≤ 5 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.