Name : Quick-DNA Fungal/Bacterial Microprep Kit

Cat # : D6007    

Size : 50 Preps

Brand ：Zymo Research

_d6007_quick-dna_fungalbacterial_microprep_kit.pdf

HIGHLIGHTS

Boost Detection: Included BashingBeads ensure complete lysis of tough-to-lyse samples.

Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.

Simple: Fastest workflow (≤ 20 minutes).

DESCRIPTION

The Quick-DNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.

TECHNICAL SRECIFICATIONS

Applicable For

All sensitive downstream applications such as qPCR and Next-Generation Sequencing.

Elution Volume

≥ 10 µl

Equipment

Microcentrifuge, vortex, cell disruptor/pulverizer

Processing Time

≤ 15 minutes

Processing Volume

≤20 mg fungi or bacteria (wet weight), 2x108 bacterial cells, 2x107 yeast cells, or 2x106 mammalian cells

Purity

Typical A260/A280 & A260/A230 ≥ 1.8

Sample Source

Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled.

Size Range

Capable of recovering genomic DNA sized fragments from up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered

Type

Total DNA

Yield

≤ 5 µg total DNA

Supplemental Info

Purpose of Genomic DNA Extraction & Tips For Success