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产品名称 Zymolyase-20T / Zymolyase-100T
货号: ​07663-91/07663-04;07665-55/07665-84 规格:
品牌: 热销产品 价格:
产品分类: 热销产品 研究领域:
CAS编号: 分子量:
分子式: 别名:
储存条件: 运输条件:
货期: 应用:
说明书: 备注:
产品描述

zymolyase-100t.jpg

产品名称:Zymolyase-20T / Zymolyase-100T

品牌:Nacalai


Zymolyase.pdf


Zymolyase®, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase® is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit


Product Information

There are two preparations of Zymolyase®, Zymolyase®-20T and Zymolyase®-100T, having lytic activity of 20,000 units/g and 100,000 units/g respectively. Zymolyase®-20T is ammonium sulfate precipitate while Zymolyase®-100T is a further purified preparation by affinity chromatography(9). Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions(6-8). Further informations related to Zymolyase® can be obtained in the reference section below(12-16).


Product Name

Zymolyase®-20T

Zymolyase®-100T

Form

Lyophilized Powder

Purification

Ammonium Sulfate Precipitation

Affinity Chromatography

Activity

20,000 units/g

100,000 unites/g

Essential enzyme

β-1,3-glucan laminaripentaohydrolase

Other activities
contained (※1)

β-1,3-glucanase

approx. 1.5 x 106 units/g

approx. 1.0 x 107 units/g

protease

approx. 1.0 x 104 units/g

approx. 1.7 x 104 units/g

mannanase

approx. 1.0 x 106 units/g

approx. 6.0 x 104 units/g

Contaminants

Amylase

Trace amount

Non detectable

Xylanase

Phosphatase

Optimum pH and Temp.

pH7.5, 35°C (for lysis of viable yeast cells)

pH6.5, 45°C (for hydrolysis of yeast glucan)

Stability

2°C

No loss of activity was found after storage for 1 year

Heat stability

30°C

70% of the lytic activity is lost after storage for 3 months

90% of the lytic activity is lost after storage for 3 months

60°C

Lytic activity is lost on incubation for 5 minutes

Specificity (Lytic Spectrum)

Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwanniomyces, etc.


(※1) See reference, Kitamura, K., Kaneko, T., Yamamoto, Y., J. Gen. Appl. Microbiol., 18, 57 (1972) as to the definition of each enzyme units.


Ordering Information


Product Name

Storage

Product No.

PKG Size

Price

Zymolyase®-20T

R

07663-91

1 g

请咨询客服

07663-04

10 g

Zymolyase®-100T

R

07665-55

500 mg

请咨询客服

07665-84

5 g


Zymolyase® is a registered trademark of Kirin Holdings Company, Limited

(Storage) R: Refrigerator






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