维百奥生物现货销售Sunrise Science完全混合氨基酸酵母培养基及添加物

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品名：Sunrise Science完全混合氨基酸酵母培养基及添加物

品牌：Sunrise Science

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●Recipe in mg/L:

Adenine hemisulfate (10 mg/L)；L-Arginine (50 mg/L)；L-Aspartic acid (80 mg/L)；L-Histidine hydrochloride monohydrate (20 mg/L)；L-Isoleucine (50 mg/L)；L-Lysine hydrochloride (50 mg/L)；L-Methionine (20 mg/L)；L-Phenylalanine (50 mg/L)；L-Threonine (100 mg/L)；L-Tryptophan (50 mg/L)；L-Tyrosine (50 mg/L)；L-Valine (140 mg/L)；Uracil (20 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 0.69

Storage Temperature: Room Temp

●Recipe in mg/L:

Adenine hemisulfate (10 mg/L))；L-Arginine (50 mg/L)；L-Aspartic acid (80 mg/L)；L-Isoleucine (50 mg/L)；L-Leucine (100 mg/L)；L-Lysine hydrochloride (50 mg/L)；L-Methionine (20 mg/L)；L-Phenylalanine (50 mg/L)；L-Threonine (100 mg/L)；L-Tryptophan (50 mg/L)；L-Tyrosine (50 mg/L)；L-Valine (140 mg/L)；Uracil (20 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 0.77

Storage Temperature: Room Temp

●Recipe in mg/L:

Adenine hemisulfate (10 mg/L);L-Arginine (50 mg/L);L-Aspartic acid (80 mg/L);L-Histidine hydrochloride monohydrate (20 mg/L);L-Isoleucine (50 mg/L);L-Leucine (100 mg/L);L-Lysine hydrochloride (50 mg/L);L-Methionine (20 mg/L);L-Phenylalanine (50 mg/L);L-Threonine (100 mg/L);L-Tyrosine (50 mg/L);L-Valine (140 mg/L);Uracil (20 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 0.74

Storage Temperature: Room Temp

●Recipe in mg/L:

Adenine hemisulfate (10 mg/L);L-Arginine (50 mg/L);L-Aspartic acid (80 mg/L);L-Histidine hydrochloride monohydrate (20 mg/L);L-Isoleucine (50 mg/L);L-Leucine (100 mg/L);L-Lysine hydrochloride (50 mg/L);L-Methionine (20 mg/L);L-Phenylalanine (50 mg/L);L-Threonine (100 mg/L);L-Tryptophan (50 mg/L);L-Tyrosine (50 mg/L);L-Valine (140 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 0.77

Storage Temperature: Room Temp

●Recipe in mg/L:

Salts, Vitamins, Minerals & Trace Elements

Biotin (0.002 mg/L);Boric acid (0.5 mg/L);Calcium chloride dihydrate (100 mg/L);Copper (II) sulfate pentahydrate (0.04 mg/L);Folic acid (0.002 mg/L);Inositol (In Base Formula) (2 mg/L);Iron (III) chloride (0.2 mg/L);Magnesium sulfate anhydrous (500 mg/L);Manganese sulfate monohydrate (0.4 mg/L);Niacin (0.4 mg/L);4-Aminobenzoic acid (PABA) (In Base Formula) (0.2 mg/L);D-Pantothenic acid hemicalcium salt (0.4 mg/L);Potassium iodide (0.1 mg/L);Potassium phosphate monobasic anhydrous (1000 mg/L);Pyridoxine hydrochloride (0.4 mg/L);Riboflavin (0.2 mg/L);Sodium chloride (100 mg/L);Sodium molybdate (0.2 mg/L);Thiamine hydrochloride (0.4 mg/L);Zinc sulfate monohydrate (0.4 mg/L)

Amino Acids & Supplements

Adenine hemisulfate (10 mg/L);L-Arginine (50 mg/L);L-Aspartic acid (80 mg/L);L-Histidine hydrochloride monohydrate (20 mg/L);L-Isoleucine (50 mg/L);L-Leucine (100 mg/L);L-Lysine hydrochloride (50 mg/L);L-Methionine (20 mg/L);L-Phenylalanine (50 mg/L);L-Threonine (100 mg/L);L-Tyrosine (50 mg/L);L-Valine (140 mg/L)

Nitrogen

Ammonium sulfate (5000 mg/L)

Sugar

Glucose (20000 mg/L)

●Instructions

Add at suggested g/L to deionized water. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 27.42

Storage Temperature: Room Temp

●Recipe in mg/L:

Adenine hemisulfate (21 mg/L);L-Alanine (85.6 mg/L);L-Arginine (85.6 mg/L);L-Asparagine monohydrate (85.6 mg/L);L-Aspartic acid (85.6 mg/L);L-Cysteine hydrochloride monohydrate (85.6 mg/L);L-Glutamic acid (85.6 mg/L);L-Glutamine (85.6 mg/L);L-Histidine hydrochloride monohydrate (85.6 mg/L);Inositol (in AA/Supplement Mix) (85.6 mg/L);L-Isoleucine (85.6 mg/L);L-Leucine (173.4 mg/L);L-Lysine hydrochloride (85.6 mg/L);L-Methionine (85.6 mg/L);4-Aminobenzoic acid (PABA) (In AA/Supplement Mix) (8.6 mg/L);L-Phenylalanine (85.6 mg/L);L-Proline (85.6 mg/L);L-Threonine (85.6 mg/L);L-Tryptophan (85.6 mg/L);L-Tyrosine (85.6 mg/L);L-Valine (85.6 mg/L);Uracil (85.6 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes. To prepare plates, autoclave agar separately and add to sterile medium, or add agar to liquid medium and adjust to pH 5.8-6.0 before autoclaving.

Suggested g/L: 1.83

Storage Temperature: 2-8 C

●Recipe in mg/L:

Adenine hemisulfate (10 mg/L);L-Arginine (50 mg/L);L-Aspartic acid (80 mg/L);L-Histidine hydrochloride monohydrate (20 mg/L);L-Isoleucine (50 mg/L);L-Leucine (100 mg/L);L-Methionine (20 mg/L);L-Phenylalanine (50 mg/L);L-Threonine (100 mg/L);L-Tryptophan (50 mg/L);L-Tyrosine (50 mg/L);L-Valine (140 mg/L);Uracil (20 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 0.74

Storage Temperature: Room Temp

●Recipe in mg/L:

Adenine hemisulfate (10 mg/L);L-Arginine (50 mg/L);L-Aspartic acid (80 mg/L);L-Histidine hydrochloride monohydrate (20 mg/L);L-Isoleucine (50 mg/L);L-Lysine hydrochloride (50 mg/L);L-Methionine (20 mg/L);L-Phenylalanine (50 mg/L);L-Threonine (100 mg/L);L-Tryptophan (50 mg/L);L-Tyrosine (50 mg/L);L-Valine (140 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 0.67

Storage Temperature: Room Temp

●Recipe in mg/L:

Adenine hemisulfate (10 mg/L);L-Arginine (50 mg/L);L-Aspartic acid (80 mg/L);L-Histidine hydrochloride monohydrate (20 mg/L);L-Isoleucine (50 mg/L);L-Leucine (100 mg/L);L-Lysine hydrochloride (50 mg/L);L-Methionine (20 mg/L);L-Phenylalanine (50 mg/L);L-Threonine (100 mg/L);L-Tyrosine (50 mg/L);L-Valine (140 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 0.72

Storage Temperature: Room Temp

●Recipe in mg/L:

Adenine hemisulfate (10 mg/L);L-Aspartic acid (80 mg/L);L-Histidine hydrochloride monohydrate (20 mg/L);L-Isoleucine (50 mg/L);L-Leucine (100 mg/L);L-Lysine hydrochloride (50 mg/L);L-Methionine (20 mg/L);L-Phenylalanine (50 mg/L);L-Threonine (100 mg/L);L-Tryptophan (50 mg/L);L-Tyrosine (50 mg/L);L-Valine (140 mg/L);Uracil (20 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 0.74

Storage Temperature: Room Temp

●Recipe in mg/L:

Biotin (0.002 mg/L);Boric acid (0.5 mg/L);Calcium chloride dihydrate (100 mg/L);Copper (II) sulfate pentahydrate (0.04 mg/L);Folic acid (0.002 mg/L);Inositol (In Base Formula) (2 mg/L);Iron (III) chloride (0.2 mg/L);Magnesium sulfate anhydrous (500 mg/L);Manganese sulfate monohydrate (0.4 mg/L);Niacin (0.4 mg/L);4-Aminobenzoic acid (PABA) (In Base Formula) (0.2 mg/L);D-Pantothenic acid hemicalcium salt (0.4 mg/L);Potassium iodide (0.1 mg/L);Potassium phosphate monobasic anhydrous (1000 mg/L);Pyridoxine hydrochloride (0.4 mg/L);Riboflavin (0.2 mg/L);Sodium chloride (100 mg/L);Sodium molybdate (0.2 mg/L);Thiamine hydrochloride (0.4 mg/L);Zinc sulfate monohydrate (0.4 mg/L)

●Instructions

Commonly added at the suggested g/L to a nitrogen source, sugar, and amino acid mixture. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 1.71

Storage Temperature: Room Temp

●Recipe in mg/L:

Biotin (0.002 mg/L);Boric acid (0.5 mg/L);Calcium chloride dihydrate (100 mg/L);Folic acid (0.002 mg/L);Inositol (In Base Formula) (2 mg/L);Niacin (0.4 mg/L);4-Aminobenzoic acid (PABA) (In Base Formula) (0.2 mg/L);D-Pantothenic acid hemicalcium salt (0.4 mg/L);Potassium iodide (0.1 mg/L);Potassium phosphate monobasic anhydrous (1000 mg/L);Pyridoxine hydrochloride (0.4 mg/L);Riboflavin (0.2 mg/L);Sodium chloride (100 mg/L);Thiamine hydrochloride (0.4 mg/L)

●Instructions

Commonly added at the suggested g/L to a sugar and an amino acid mixture. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 6.20

Storage Temperature: Room Temp

●Recipe in mg/L:

Boric acid (0.5 mg/L);Calcium chloride dihydrate (14.7 mg/L);Citric acid trisodium dihydrate (1 mg/L);Copper (II) sulfate pentahydrate (0.04 mg/L);Inositol (In Base Formula) (10 mg/L);Iron (III) chloride (0.138 mg/L);Magnesium chloride anhydrous (492 mg/L);Manganese sulfate monohydrate (0.4 mg/L);Niacin (10 mg/L);D-Pantothenic acid hemicalcium salt (1 mg/L);Potassium chloride (1000 mg/L);Potassium iodide (0.1 mg/L);Potassium phthalate monobasic (3000 mg/L);Sodium molybdate (0.04 mg/L);Sodium phosphate dibasic anhydrous (2200 mg/L);Sodium sulfate anhydrous (40 mg/L);Zinc sulfate monohydrate (0.4 mg/L)

●Instructions

Add at suggested g/L to deionized water. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 31.77

Storage Temperature: Room Temp

●Recipe in mg/L:

Salts, Vitamins, Minerals & Trace Elements

Calcium chloride dihydrate (26.4 mg/L);Citric acid (5136 mg/L);Citric acid trisodium dihydrate (9480 mg/L);Iron (II) sulfate heptahydrate (5.6 mg/L);Magnesium chloride hexahydrate (400 mg/L);Potassium phosphate monobasic anhydrous (1000 mg/L);Sodium chloride (14560 mg/L)

mino Acids & Supplements

L-Alanine (224 mg/L);L-Arginine (316 mg/L);L-Asparagine monohydrate (176 mg/L);L-Aspartic acid (144 mg/L);L-Cysteine hydrochloride monohydrate (548 mg/L);L-Glutamic acid potassium salt monohydrate (1344 mg/L);L-Glutamine (712 mg/L);L-Glycine (176 mg/L);L-Histidine hydrochloride monohydrate (110 mg/L);L-Isoleucine (224 mg/L);L-Leucine (462 mg/L);L-Methionine (136 mg/L);L-Phenylalanine (208 mg/L);L-Proline (696 mg/L);L-Serine (354 mg/L);L-Threonine (244 mg/L);L-Tryptophan (100 mg/L);L-Tyrosine (218 mg/L);L-Valine (322 mg/L)

Other Additives

Methyl-b-cyclodextrin (2000 mg/L);RPMI 1640-Glutamine-Amino acids-Glucose (2000 mg/L)

●Instructions

For a 1X solution, add pouch contents to 1000mL deionized water. For a 2X solution, add pouch contents to 500mL deionized water. Adjust pH to 4.75 with 6N NaOH and filter sterilize. NOTE: This optimized formulation contains several highly-hydrated ingredients. Some clumping of powder may be expected and should be easily dispersed when stirred to dissolve. Store powder and prepared medium at 2C-8C.

Suggested g/L: 41.32

Storage Temperature: 2-8 C

●Recipe in mg/L:

Adenine hemisulfate (10 mg/L);L-Arginine (50 mg/L);L-Aspartic acid (80 mg/L);L-Isoleucine (50 mg/L);L-Lysine hydrochloride (50 mg/L);L-Methionine (20 mg/L);L-Phenylalanine (50 mg/L);L-Threonine (100 mg/L);L-Tyrosine (50 mg/L);L-Valine (140 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 0.60

Storage Temperature: Room Temp

Zymolyase®, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls and can therefore be used to produce protoplasts or spheroplasts of various strains. An essential enzyme for the lytic activity of Zymolyase® is beta-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers at beta-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit.

Zymolyase®-20T is an ammonium sulfate precipitate with a lytic activity of 20,000 units/g. Lytic activity varies depending on yeast strain, growth stage of yeast, and culture conditions.

●Recipe in mg/L:

Salts, Vitamins, Minerals & Trace Elements

Calcium chloride dihydrate (26 mg/L);Citric acid (5140 mg/L);Citric acid trisodium anhydrous (8320 mg/L);Iron (II) sulfate hydrate (3 mg/L);Magnesium chloride anhydrous (190 mg/L);Potassium phosphate monobasic anhydrous (1000 mg/L);Sodium chloride (14560 mg/L)

Amino Acids & Supplements

L-Cysteine hydrochloride monohydrate (527 mg/L)

Rich Supplements

Casamino acids (5000 mg/L);Tryptone (Casein Peptone) (200 mg/L)

Other Additives

Methyl-b-cyclodextrin (2000 mg/L)

●Instructions

Mix pouch contents with 375 mL diH2O and 125 mL RPMI1640+GlutaMAX. Adjust pH to 4.75 with 6N NaOH. Filter sterilize and store at 4C.

Suggested g/L: 36.96

Storage Temperature: Room Temp

Molecular Formula: C4H4N2O2

Molecular Weight: 112.09

CAS Number: 66-22-8

Protect from moisture, and avoid prolonged exposure to air. Store tightly closed in a dry location.

Storage Temperature: Room Temp

●Recipe in mg/L:

Adenine hemisulfate (10 mg/L);L-Arginine (50 mg/L);L-Aspartic acid (80 mg/L);L-Histidine hydrochloride monohydrate (20 mg/L);L-Isoleucine (50 mg/L);L-Lysine hydrochloride (50 mg/L);L-Methionine (20 mg/L);L-Phenylalanine (50 mg/L);L-Threonine (100 mg/L);L-Tyrosine (50 mg/L);L-Valine (140 mg/L);Uracil (20 mg/L)

●Instructions

Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes.

Suggested g/L: 0.64

Storage Temperature: Room Temp