Elastase

货号：LS002292

Source: Porcine Pancreas

CAS: 39445-21-1  

EC: 3.4.21.36

I.U.B.: 3.4.21.36

【商品信息】

I.U.B.: 3.4.21.36

Porcine pancreatic elastase has a molecular weight of 25.9 kDa, and a pH optimum of 8.5.  While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Soybean trypsin inhibitor and kallikrein inhibitor suppress proteolytic but not elastolytic activity.  Elastase is assayed using a method adapted from that of Feinstein et al., Biochem. Biophys. Res. Comm., 50, 1020 (1973) and using the more soluble substrate of Bieth et al., Biochem. Med., 11, 350 (1974).

Stability/Storage: Elastase is unstable at pH ≤ 3.5. When stored as a dry powder the enzyme is stable for 6-12 months at 2-8°C.  Elastase product codes: ES and ESL have poor solubility at neutral pH and at concentrations greater than 0.25%.  It is helpful to make primary solutions in KCl or alkaline buffers before diluting into the reaction mixtures or media, compensating for ionic strength or pH changes.  Stable at pH 4.0-10.4.

Technical Notes: 1 SucAla3NA unit is approximately equivalent to 6 elastin digestion units. Aqueous liquid suspensions should be aseptically handled to avoid bacterial contamination. Due to the viscous nature of the aqueous suspension (Code: ES) the vial should be rinsed to recover contents.

Related Products:

Collagenase (CLS1-4/CLSPA)

Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)

Hyaluronidase (HSE/HSEP)

Neutral Protease (Dispase, NPRO)

Papain (PAP/PAPL/PAP2)

Pepsin (PM)

Proteinase K (PROK)

Trypsin (TL/TRL/TRL3/TRLS/TRTPCK)

Trypsin Inhibitors (LBI/OI/SI/SIC)

Cell Isolation Optimizimg System (CIT)

Hepatocyte Isolation System (HIS)

Neonatal Cardiomyocyte Isolation System (NCIS)

Papain Dissociation System (PDS/PDS2)

Unit Definition

One Unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25°C, pH 8.0.

【价格信息】

LS006363

5 mg

$82.00

LS006365

20 mg

$240.00

LS006367

Bulk

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LS002290

25 mg

$55.00

LS002292

100 mg

$156.00

LS002294

1 gm

$1,125.00

LS002298

Bulk

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LS002274

25 mg

$52.00

LS002279

100 mg

$141.00

LS002280

1 gm

$1,065.00

LS002276

Bulk

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