TRIzol® Reagent

货号: 15596018CN

【价格信息】

15596018CN 200 mL

价格 4,740.00

15596026CN 100 mL

价格 2,663.00

【商品信息】

TRIzol Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.

Key features of TRIzol Reagent include:

• Permits the isolation of RNA, DNA, and protein from the same sample

• Offers superior lysis capability, even with difficult sample types

• Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria

Reliably purify RNA from multiple sample volumes and sources

TRIzol Reagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 106), as well as with large quantities of tissue (≥1 g) and cells (>107), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin. TRIzol Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. The simplicity of the TRIzol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol Reagent is free of protein and DNA contamination.

Formulated for isolation of multiple molecular targets

TRIzol Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.

规格

分离技术：有机提取

Purification Time：1小时

样品类型：细菌, 血液, 细胞, 植物样本, 组织, 病毒样本, 酵母

最终产品类型：总 RNA、转录组 RNA、微量 RNA

高通量能力：不兼容高通量应用（手动）

适用于（应用）：实时荧光定量 PCR (qPCR)，逆转录酶 PCR (RT-PCR)，cDNA 文库构建，核酸酶保护测定，Northern 印迹，克隆

产品线：TRIzol™

数量：200 次制备

规格：刻度可读

运输条件：室温

原始材料量：组织：≤1 g 细胞：≤1 x 107

容积（公制）：200 mL

内容与储存

一瓶 TRIzol 试剂。在室温下储存。