Name ：Zymoclean Gel RNA Recovery Kit

Cat # ：R1011                            

Size ：50 Preps

Brand：Zymo Research

_r1011_zymoclean_gel_rna_recovery_kit.pdf

HIGHLIGHTS

Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.

Concentrated: Up to 10 µg sample in ≥ 6 µl elution.

High Recovery: ≥ 80% recovery for RNA > 500 nt

DESCRIPTION

The Zymoclean Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Zymo-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).

TECHNICAL SRECIFICATIONS

Equipment

Microcentrifuge, 37°C-65°C heat source

Purity

RNA is ready for all subsequent analysis and molecular manipulation

Sample Source

Single- or double-stranded RNA fragments (≥200 nt) resolved in TAE TBE, and MOPS buffered agarose gels. Compatible with formaldehyde to 2.0% (final conc.)

Size Range

RNA fragments ≥500 nt (≥80% recovery)

Yield

10 µg RNA (binding capacity), ≥6 µl (elution volume)