ZymoPURE II Plasmid Maxiprep Kit

Cat # Name                                                  Size

D4203 ZymoPURE II Plasmid Maxiprep Kit 20 Preps.

D4202 ZymoPURE II Plasmid Maxiprep Kit 10 Preps.

Brand ：Zymo Research

_d4202_d4203_zymopure_ii_plasmid_maxiprep.pdf

HIGHLIGHTS

Perform plasmid maxipreps in only 16 minutes using a simple spin-column protocol.

Purify up to 3 mg of highly concentrated plasmid DNA directly from a spin-column.

Eluted plasmid DNA is Endotoxin-free and Transfection-Ready.

DESCRIPTION

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 3 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Maxiprep kit can process up to 150 ml of culture in less than 18 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with either a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 µg/µl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. 

TECHNICAL SRECIFICATIONS

Applicable For

Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications.

Binding Capacity

3.0 mg

Culture Input

≤ 150 ml

Elution Volume

≥ 300 µl

Endotoxin Levels

≤ 0.025 EU/µg of Plasmid DNA

Equipment

Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge.

Format

Spin-Column

Processing Time

≤ 18 min

Purity

Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0

Recommended Sample Volume

≤ 150 ml

Size Range

Up to 200 kb

Yield

Up to 3.0 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 150 ml of overnight culture grown in LB for high copy number plasmids are 600 – 1,200 µg.

Supplemental Info

How to Increase Plasmid Yield

DOs and DON'Ts of Plasmid Purification