FLT3 Kinase Assay Kit

Catalog # 79797

Size: 96 reactions

Description

The FLT3 Kinase Assay Kit is designed to measure FLT3 kinase activity for screening and profiling applications using ADP-Glo® 

Kinase Assay as a detection reagent.

MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase 

activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated

 version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not

 suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.

Need us to run inhibitor screens or profile your compounds against FLT3? Check out our Kinase Screening Services.

SynonymsFms-Related Tyrosine Kinase 3 Ligand, FLT3L, CD135L

ACHE Colorimetric Assay Kit

Catalog # 82281

Size: 96 reactions

Description

The ACHE Colorimetric Assay Kit is designed to measure the activity of ACHE (acetylcholinesterase) for screening and profiling

 applications. The ACHE colorimetric reaction is based on a modified Ellman’s method, using the alternative substrate acetylthio

choline iodide (ATCI) and 5,5’-dithio-bis-2-nitrobenzoic acid (DTNB) to quantify amount of thiocholine produced from the 

hydrolysis of ATCI by ACHE. This assay kit comes in a convenient 96-well format, with purified ACHE enzyme (amino acids 

32-614), ATCI, DTNB and assay buffer for 100 enzyme reactions. The absorption intensity of DTNB adduct (λ=410 nm) is used 

to measure the amount of thiocholine product generated, which is proportional to ACHE activity.

Need us to run inhibitor screens or profile your compounds against ACHE? Check out our Metabolic Enzyme Screening Services.

SynonymsAcetylcholine receptor subunit epsilon, CHRNE, ACHRE

Cathepsin D Inhibitor Screening Assay Kit

Catalog #82141

Description

The Cathepsin D Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin D for screening and 

profiling applications. The Cathepsin D assay kit comes in a convenient 96-well or 384-well format, with enough recombinant

 human Cathepsin D (amino acids 21-412), its substrate, and Cathepsin buffer for 96 or 384 reactions. This kit contains Pepstatin

 A as internal control.

Figure 1: Illustration of the assay principle.

The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent substrate from the quen

cher. Fluorescence intensity increases proportionally to the activity of the protease.

Need us to run inhibitor screens or profile your compounds against Cathepsin D? Check out our Protease Screening Services.

Chemi-Verse™ YES1 Kinase Assay Kit

Catalog #82556

Size: 96 reactions

Description

The Chemi-Verse™ YES1 Kinase Assay Kit is designed to measure YES1 (Yamaguchi sarcoma viral oncogene homolog 1) tyrosine 

kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient

 96-well format, with enough purified YES1, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Need us to run inhibitor screens or profile your compounds against YES1? Check out our Kinase Screening Services.

SynonymsProto-oncogene c-Yes, p61-Yes, Tyrosine-protein kinase Yes, YES1

COX1 Inhibitor Screening Assay Kit

Catalog #82203

Size: 96 reactions

Description

The COX1 Inhibitor Screening Assay Kit is designed to measure COX1 (cyclooxygenase 1) activity for screening and profiling

 applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant COX1, Amplex™ Red,

 Arachidonic Diluent, COX Assay Buffer, and 100% ethanol for 100 enzyme reactions.

STK3 (MST2) Kinase Assay Kit

Catalog #82140

Size: 96 reactions

Description

The STK3 (MST2) Kinase Assay Kit is designed to measure STK3 (serine/threonine-protein kinase 3) kinase activity for screening 

and profiling applications using Kinase-Glo® MAX as a detection reagent. The assay kit comes in a convenient 96-well format,

 with enough purified recombinant STK3 (MST2) kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

SynonymsKRS1; MST2; FLJ90748

TWO-Step Luciferase (Firefly & Renilla) Assay System

Catalog #60683

Description

The TWO-Step Luciferase (Firefly & Renilla) Assay System is designed for high throughput, rapid quantitation of both Firefly and 

Renilla luciferases in a single mammalian cell culture sample. It comes with enough reagents for 100 assays of 100 μl each in a 

96-well plate.

This assay system has several relevant features:

Sensitive – highly sensitive detection of Firefly and Renilla luciferase activity.

Stable – the luciferase signal output is stable for more than one hour, providing incubation time flexibility.

High-throughput – its simple homogeneous protocol minimizes handling steps and supports high-throughput screening 

applications.

Compatibility – works with a variety of common media containing up to 10% serum and phenol red.