Percoll™ density gradient media

17089101

Percoll - 1 L

1 L

In stock

111 available

17089102

Percoll 250 mL

250 mL

In stock

47 available

17089109

Percoll 6 × 1 L

6 × 1 L

In stock

6 available

A low-viscosity, non-toxic medium suitable for density gradient centrifugation of cells, viruses and subcellular particles.

Overview

A low-viscosity, non-toxic medium suitable for density gradient centrifugation of cells, viruses and subcellular particles.

Composed of colloidal silica coated with polyvinylpyrrolidone (PVP), which gives low osmolality and low viscosity.

For separation of cells, subcellular particles and larger viruses (down to ~ 70S) under gentle conditions which preserve viability 

and morphological integrity.

Non-toxic to cells.

Adjustable to physiological ionic strength and pH.

Gradients can either be pre-formed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor.

Gradients are iso-osmotic throughout and cover a range of densities up to 1.3 g / mL.

Quick and easy

Percoll is a low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses. The low

 viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces

 (200 to 1000 × g). The medium is available in easy-to-open, resealable 250 mL and 1 L bottles.

Other attributes

Percoll consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). Free PVP is 

present at only 1% to 2%. Percoll is non-toxic, almost chemically inert and does not adhere to membranes. Percoll gradients

 can be formed within the density range of 1.0 to 1.3 g/mL, and are iso-osmotic throughout.

Percoll can be stored unopened at room temperature for five years. At -20°C, it can only be stored for up to six months.

If stored at -20°C, gradients form upon thawing, necessitating a mixing of the bottle before use. Preformed gradients can be 

stored for weeks without a change in gradient shape, provided that the gradient remains unfrozen.

Percoll can be buffered within the pH range 5.5 to 10.0 without any changes in properties. If pH drops below 5.5, gelling may

 occur. Gelling can also be caused by the presence of divalent cations, an effect which is exacerbated by elevated temperatures.

Undiluted Percoll can be resterilized by autoclaving for 30 min at 120°C.

Ficoll-Paque™ PLUS density gradient media

A recognized worldwide standard sterile medium designed for isolating human lymphocytes for in vitro studies.

17144003

Ficoll-Paque PLUS

6 × 500 mL

In stock

214 available

17144002

Ficoll-Paque PLUS

6 x 100 mL

In stock

192 available

Overview

A recognized worldwide standard sterile medium designed for isolating human lymphocytes for in vitro studies.

Ready to use sterile medium for high yield isolation of human lymphocytes from peripheral blood.

Maintains viability and representative distribution of B and T lymphocytes.

Low levels of endotoxin (<0.12 EU/mL).

Recognized standard

Ficoll-Paque PLUS (density 1.077 g/mL) is a recognized standard in laboratories worldwide for the isolation of mononuclear 

cells for analytical research studies.

Ficoll-Paque™ PREMIUM density gradient media

17544202

Ficoll-Paque PREMIUM

1.078 g/ml

6 x 100 mL

In stock

69 available

17544203

Ficoll-Paque PREMIUM

1.078 g/ml

6 × 500 mL

In stock

68 available

17544652

Ficoll-Paque PREMIUM 1.073

1.074 g/ml

6 x 100 mL

In stock

49 available

17544602

Ficoll-Paque PREMIUM 1.084

1.085 g/ml

6 x 100 mL

In stock

14 available

Ready-to-use sterile density gradient media designed for the preparation of mononuclear cells from peripheral blood, bone

 marrow and cord blood.

Overview

Ready-to-use sterile density gradient media designed for the preparation of mononuclear cells from peripheral blood, bone 

marrow and cord blood.

Manufactured under a Quality Management System certified to ISO 13485.

Low levels of endotoxin (< 0.12 EU/mL) secured and tested.

Cytotoxicity grade 0.

Choice of densities: 1.077, 1.084, and 1.073 g/mL.

A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

Ficoll-Paque PREMIUM vs Ficoll-Paque PLUS

All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management

 System certified to ISO 13485.

How it's used

Ficoll-Paque PREMIUM: Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and 

umbilical cord blood.

Ficoll-Paque PREMIUM 1.084: Can be used for isolating a broad range of human mononuclear cells including those of a higher

 density and for separating blood cells from mice or rats.

Ficoll-Paque PREMIUM 1.073: Can be used when isolating lower-density human mononuclear cells (e.g. mesenchymal stromal

 cells or monocytes).