RPMI-1640 Medium

30-2001 

500 mL

RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 

1500 mg/L sodium bicarbonate, for use in incubators using 5% CO2 in air. Additional sodium bicarbonate may be required for 

use in incubators containing higher percentages of CO2. It has applications for cell culture, cell growth, and viability.

Product category

Media

Formulation

Contains 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate.

Comments

NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium 

bicarbonate may be required for use in incubators containing higher percentages of CO2.

Applications

Cell culture

Cell growth and viability

Volume

500 mL

Storage conditions

2°C to 8°C