HyClone Minimal Essential Medium (MEM) variations: Liquid

SH30265.01

500 mL

MEM Alpha modification, with L-glutamine, ribo- and deoxyribonucleosides

HyClone MEM media promotes cell growth in research and biomanufacturing cell culture processes.

Overview

HyClone MEM liquid media supports cell growth in research, upstream process development, and cell culture manufacturing 

processes.

Minimum essential medium efficacy and homogeneity is tested with Cytiva HyClone sera.

MEM composition includes vitamins, minerals, and amino acids vital to supporting mammalian cell culture and promoting cell

 growth. There are several MEM media options to support cell culture process development. Formulations of HyClone cell 

culture MEM media are available with or without L-glutamine. The MEM alpha modification includes the additional components

 such as non-essential amino acids, ascorbic acid, biotin, vitamin B12, lipoic acid, sodium pyruvate, ribonucleosides and

 deoxyribonucleosides. Improved MEM, or Richter’s modification, is also available with increased concentrations of certain

 amino acids, vitamins, and metabolites. In many MEM media formulations, phenol red is added as a pH indicator. All available 

MEM media contain a sodium bicarbonate buffer with some also containing EBSS to maintain optimal culture pH.

HyClone MEM raw material components are screened through strict quality control testing for lot-to-lot consistency. MEM

 liquid media products are hydrated using purified water and undergo sterile filtration.

HyClone Dulbecco's Modified Eagle Medium (DMEM) with low glucose: Liquid

SH30021.01

500 mL

DMEM/Low glucose with L-glutamine, sodium pyruvate

Overview

Supports cell growth in research and biomanufacturing cell culture processes.

Raw material components screened through strict quality control testing for lot-to-lot consistency.

Product efficacy and homogeneity tested with Cytiva HyClone sera.

Products are hydrated using purified process water and undergo sterile filtration.

HyClone Dulbecco's Modified Eagle Medium (DMEM) with high glucose: Liquid

SH30243.02

1000 mL

DMEM/High glucose with L-glutamine, sodium pyruvate

Overview

HyClone DMEM high glucose liquid media supports cell growth in research, upstream process development, and cell culture

 manufacturing processes.

DMEM high glucose medium efficacy and homogeneity is tested with HyClone sera.

DMEM media contain increased concentrations of vitamins, minerals, and amino acids compared to traditional basal media. 

DMEM medium composition also includes ferric nitrate, making this cell culture medium an option for certain cell lines requiring

 iron for cell growth. HyClone DMEM media preparations are available in high or low glucose formulations, as well as with or

 without L-glutamine, magnesium, and sodium pyruvate. DMEM formulation options may also include phenol red as a pH

 indicator. All available DMEM media products contain a sodium bicarbonate buffer with or without HEPES to maintain optimal 

culture pH.

HyClone DMEM raw material components are screened through strict quality control testing for lot-to-lot consistency. DMEM

 liquid media products are hydrated using purified water and undergo sterile filtration.

HyClone RPMI 1640 media: Liquid

SH30809.01

Supports cell growth in research and biomanufacturing cell culture processes.

HyClone Leibovitz L-15 medium: Liquid

SH30525.01

500 mL

Overview

Supports cell growth in research and biomanufacturing cell culture processes.

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities.

Raw material components screened through strict quality control testing for lot-to-lot consistency.

Products are hydrated using purified process water and undergo sterile filtration.

HyClone SFX-Insect cell culture media

SH30278.02

1000 mL

SFX-Insect liquid medium with L-glutamine, sodium bicarbonate - 1000 mL

Serum-free medium supports recombinant protein production with multiple insect cell lines.

Designed for high cell yield and recombinant protein production.

Complexed lipids for enhanced stability.

Supports direct and sequential adaptation

High performance growth in multiple culture systems including high density bioreactors.

Manufactured using ISO 9001 and ISO 13485 certified processes according to 21 CFR 820 medical device current good 

manufacturing practices (cGMP).

SFX-Insect medium provides excellent growth of many key insect cell lines, including Sf9, Sf21, High Five (T.ni) and D.Mel-2 cells, 

while requiring minimal adaptation. Additionally, SFX-Insect medium has been successfully tested in a variety of culture systems 

ranging from T-flasks, shaker flasks, and various traditional and disposable bioreactors. SFX-Insect medium is available as liquid 

or powder medium in bottles, bags, or custom packaging.

HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid

SH30023.01

500 mL

DMEM/F12 1:1 medium with L-glutamine, HEPES

Overview

Supports cell growth in research and biomanufacturing cell culture processes.

Raw material components screened through strict quality control testing for lot-to-lot consistency.

Product efficacy and homogeneity tested with Cytiva HyClone sera.

Products are hydrated using purified process water and undergo sterile filtration.