2X ACCM-D (-Glucose-Lys) Powder, 10 x 0.5 liter pouches

CATALOG #: 4705-300

Recipe in mg/L:

SALTS, VITAMINS, MINERALS & TRACE ELEMENTS

Calcium chloride dihydrate (26.4 mg/L)

Citric acid (5136 mg/L)

Citric acid trisodium dihydrate (9480 mg/L)

Iron (II) sulfate heptahydrate (5.6 mg/L)

Magnesium chloride hexahydrate (400 mg/L)

Potassium phosphate monobasic anhydrous (1000 mg/L)

Sodium chloride (14560 mg/L)

AMINO ACIDS & SUPPLEMENTS

L-Alanine (224 mg/L)

L-Arginine (316 mg/L)

L-Asparagine monohydrate (176 mg/L)

L-Aspartic acid (144 mg/L)

L-Cysteine hydrochloride monohydrate (548 mg/L)

L-Glutamic acid potassium salt monohydrate (1344 mg/L)

L-Glutamine (712 mg/L)

L-Glycine (176 mg/L)

L-Histidine hydrochloride monohydrate (110 mg/L)

L-Isoleucine (224 mg/L)

L-Leucine (462 mg/L)

L-Methionine (136 mg/L)

L-Phenylalanine (208 mg/L)

L-Proline (696 mg/L)

L-Serine (354 mg/L)

L-Threonine (244 mg/L)

L-Tryptophan (100 mg/L)

L-Tyrosine (218 mg/L)

L-Valine (322 mg/L)

OTHER ADDITIVES

Methyl-b-cyclodextrin (2000 mg/L)

RPMI 1640-Glutamine-Amino acids-Glucose (2000 mg/L)

Instructions

For a 1X solution, add pouch contents to 1000mL deionized water. For a 2X solution, add pouch contents to 500mL 

deionized water. Adjust pH to 4.75 with 6N NaOH and filter sterilize. NOTE: This optimized formulation contains 

several highly-hydrated ingredients. Some clumping of powder may be expected and should be easily dispersed 

when stirred to dissolve. Store powder and prepared medium at 2C-8C.

Suggested g/L: 41.32

Storage Temperature: 2-8 C

Related Categories

Nucleic Acid & Protein Purification, Lab Tools