2X ACCM-2 Powder, 10 x 0.5 liter pouches

CATALOG #: 4700-300

Recipe in mg/L:

SALTS, VITAMINS, MINERALS & TRACE ELEMENTS

Calcium chloride dihydrate (26 mg/L)

Citric acid (5140 mg/L)

Citric acid trisodium anhydrous (8320 mg/L)

Iron (II) sulfate hydrate (3 mg/L)

Magnesium chloride anhydrous (190 mg/L)

Potassium phosphate monobasic anhydrous (1000 mg/L)

Sodium chloride (14560 mg/L)

AMINO ACIDS & SUPPLEMENTS

L-Cysteine hydrochloride monohydrate (527 mg/L)

RICH SUPPLEMENTS

Casamino acids (5000 mg/L)

Tryptone (Casein Peptone) (200 mg/L)

OTHER ADDITIVES

Methyl-b-cyclodextrin (2000 mg/L)

Instructions

Mix pouch contents with 375 mL diH2O and 125 mL RPMI1640+GlutaMAX. Adjust pH to 4.75 with 6N NaOH. 

Filter sterilize and store at 4C.

Suggested g/L: 36.96

Storage Temperature: Room Temp

Related Categories

Nucleic Acid & Protein Purification, Lab Tools